Recombinant Human Insulin Protein

Product Specifications

Background

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake.

Synonyms

IDDM; IDDM1; IDDM2; ILPR; INS; IRDN; MODY10

UniProt

P01308

Accession Number

P01308

Expression System

Yeast

Tag

None

Sequence

Phe25-Lys53&Gly90-Asn110

Applications

Cell culture

Field of Research

Signal Transduction; Cardiovascular; Neuroscience; Cancer; metabolism

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is typically 0.2-1 µg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.