Recombinant Human FGF-2/FGFb Protein

Product Specifications

Background

Fibroblast growth factor 2 (FGF2) is a secreted protein and belongs to the heparin-binding growth factors family. FGF2 is produced by epithelial; tumor and other cell types. It involved in developmental processes and regulates differentiation; proliferation; and migration; FGF2 is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF2 has a high affinity for heparan sulfate and binding is a step in the FGF basic activation of FGFR tyrosine kinase.

Abbreviation

FGF-2; FGFb

Synonyms

Basic fibroblast growth factor; Bfgf; FGF-2; FGF2; FGFB; Fibroblast growth factor 2; HBGF-2; Heparin-binding growth factor 2

UniProt

P09038

Accession Number

P09038

Expression System

E.coli

Tag

N-His

Sequence

Ala 135-Ser 288

Applications

Cell culture

Field of Research

Signal Transduction; Cardiovascular; Neuroscience; Cancer; Developmental Biology; Stem cells

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

505 x 105 IU/mg.5

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.