Recombinant Human Ephrin-A4/EFNA4 Protein (Fc & His Tag)

Product Specifications

Background

Ephrin-A4 is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence; which most likely corresponds to the receptor binding domain. Ephrin-A4 consists of approximately 125 amino acids and includes four invariant cysteines; It has been shown to bind EphA2; EphA3; EphA4; EphA5; EphA6; EphA7; and EphB1. Ephrin-A4 binds promiscuously Eph receptors residing on adjacent cells; leading to contact-dependent bidirectional signaling into neighboring cells. It may play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils.

Abbreviation

Ephrin-A4; EFNA4

Synonyms

EFNA4; EPH-Related Receptor Tyrosine Kinase Ligand 4; EPLG4; Ephrin-A4; LERK-4; LERK4

UniProt

P52798

Accession Number

P52798

Expression System

HEK293 Cells

Tag

C-Fc-His

Sequence

Leu26-Gly171

Applications

ELISA

Field of Research

Cardiovascular; Neuroscience; Developmental Biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized Human EphA7-His (Cat: PKSH033689) at 2μg/ml (100 μl/well) can bind Human EFNA4-Fc-6His. The ED50 of Human EFNA4-Fc-6His is 1. 5190 ug/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.