Recombinant Human STUB1 Protein
Product Specifications
Background
E3 Ubiquitin-Protein Ligase CHIP is a cytoplasmic protein. CHIP is highly expressed in skeletal muscle, heart, pancreas, brain and placenta. CHIP interacts with the molecular chaperones Hsc70-Hsp70 and Hsp90 through its TPR domain; lead to in client substrate ubiquitylation and degradation by the proteasome. CHIP targets misfolded chaperone substrates towards proteasomal degradation. CHIP mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. CHIP plays a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. It also may regulate the receptor stability and activity through proteasomal degradation.
Abbreviation
STUB1
Synonyms
Antigen NY-CO-7; CHIP; CLL-Associated Antigen KW-8; Carboxy Terminus of Hsp70-Interacting Protein; E3 Ubiquitin-Protein Ligase CHIP; STIP1 Homology and U Box-Containing Protein 1; STUB1
UniProt
Q9UNE7
Accession Number
Q9UNE7
Expression System
E.coli
Tag
None
Sequence
Met 1-Tyr303
Field of Research
Signal Transduction; epigenetics and nuclear signal
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
34.9 kDa
Observed Molecular Weight
33 kDa
Formulation
Supplied as a 0.2 μm filtered solution of PBS, pH7.4.
Species
Human
Available Sizes
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