Recombinant Human Cyclophilin C/PPIC Protein (His&Trx Tag)
Product Specifications
Background
Cyclophilin C is an enzyme (EC 5.2.1.8) found in both prokaryotes and eukaryotes that interconverts the cis and trans isomers of peptide bonds with the amino acid proline. Proline has an unusually conformationally restrained peptide bond due to its cyclic structure with its side chain bonded to its secondary amine nitrogen. Most amino acids have a strong energetic preference for the trans peptide bond conformation due to steric hindrance, but prolines unusual structure stabilizes the cis form so that both isomers are populated under biologically relevant conditions. Proteins with prolyl isomerase activity include cyclophilin, FKBPs, and parvulin, although larger proteins can also contain prolyl isomerase domains.
Abbreviation
Cyclophilin C; PPIC
Synonyms
CYPC; Cyclophilin C; PPIC; PPIase C; Peptidyl-Prolyl Cis-Trans Isomerase C; Rotamase C
UniProt
P45877
Accession Number
P45877
Expression System
E.coli
Tag
N-Trx-His
Sequence
Lys31-Asp182
Field of Research
Signal Transduction; immunology
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
33.8 kDa
Observed Molecular Weight
34 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 10% Glycerol, pH 7.4.
Species
Human
Available Sizes
Documents
More Discoveries
Explore Other Products
Browse additional items from our catalog
