Recombinant Human Creatine Kinase, Muscle/CKM Protein (His Tag)
Product Specifications
Background
Creatine kinase M-type is also known as Creatine kinase M chain, M-CK. It is a protein that in humans is encoded by the CKM gene. It belongs to the ATP:guanido phosphotransferase family, containing 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. Creatine kinase M-type can reversibly catalyzes the transfer of phosphate between ATP and various phosphogens. It plays a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Abbreviation
CKM
Synonyms
CKM; CKMM; Creatine kinase M chain; Creatine kinase M-type; M-CK
UniProt
P06732
Accession Number
AAP35439.1
Expression System
HEK293 Cells
Tag
C-His
Sequence
Met 1-Lys381
Field of Research
Signal Transduction; Cardiovascular; Cancer; metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
44.1 kDa
Observed Molecular Weight
46 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5.
Species
Human
Available Sizes
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