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Recombinant Human Carboxypeptidase E/CPE Protein (His Tag)

Product Specifications

Background

The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. It is thought that membrane-associated CPE acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway. Defects in this protein are implicated in type II diabetes due to impaired glucose clearance and insulin resistance.

Abbreviation

Carboxypeptidase E; CPE

Synonyms

Carboxypeptidase E (CPE for short) ; Carboxypeptidase H; Enkephalin convertase; Prohormone-processing carboxypeptidase

UniProt

P16870

Accession Number

P16870

Expression System

HEK293 Cells

Tag

C-His

Sequence

Arg42-Ser453

Field of Research

Signal Transduction; Neuroscience; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

47.2 kDa

Observed Molecular Weight

50-60 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Histidine-HCl, 8% Sucrose, 2% Glycine, 20% Glycerol, 0.05% Tween 80, pH 6.5.

Species

Human

Available Sizes

Frequently Asked Questions

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