Recombinant Human Carboxypeptidase B1/CPB1 Protein (His Tag)
Product Specifications
Background
Carboxypeptidase B1 is an exopeptidase which specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. Expressed mainly in pancreas, CPB1 is a useful serum marker for acute pancreatitis and pancreatic graft rejection.Human CPB1 consists of a signal peptide, a pro region, and a mature chain. The purified rhCPB1 corresponds to the pro form, which can be activated by trypsin, the only pancreatic protease capable of generating active enzyme from the zymogen in vitro.
Abbreviation
Carboxypeptidase B1; CPB1
Synonyms
CPB; CPB1; Carboxypeptidase B; PASP; PCPB; Pancreas-Specific Protein
UniProt
P15086
Accession Number
P15086
Expression System
HEK293 Cells
Tag
C-His
Sequence
His16-Tyr417
Field of Research
Signal Transduction; metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
46.9 kDa
Observed Molecular Weight
47 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, pH 5.5.
Species
Human
Available Sizes
Curated Selection
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