Recombinant Human ATG5 Protein
Product Specifications
Background
ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.
Abbreviation
ATG5
Synonyms
APG5-like; APG5L; ASP; ATG5; Apoptosis-specific protein; Autophagy protein 5
UniProt
Q9H1Y0
Accession Number
Q9H1Y0
Expression System
E.coli
Tag
None
Sequence
Met 1-Asp275
Field of Research
Signal Transduction; Cell biology; Cardiovascular; Cancer; metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 90 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
32.4 kDa
Observed Molecular Weight
33 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 0.2M NaCl, 1mM DTT, 40% Glycerol, pH 8.0.
Species
Human
Available Sizes
Documents
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