Recombinant Human ATG10 Protein (His&T7 Tag)
Product Specifications
Background
Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme, catalyzes the transfer of ATG12 to ATG5 during in the initial stages of autophagesome formation. The heterodimer of ATG5 and ATG12 subsequntly associates non-covalently with an ATG16 multimer to generate an antophagosome. ATG10 plays a role in the conjugation of ATG12 to ATG5 by interaction with MAP1LC3A. In addition, ATG10 can diretly interact with ATG5 or ATG7.
Abbreviation
ATG10
Synonyms
APG10-Like; ATG10; Autophagy-Related Protein 10; Ubiquitin-Like-Conjugating Enzyme ATG10
UniProt
Q9H0Y0
Accession Number
Q9H0Y0
Expression System
E.coli
Tag
N-T7 & C-His
Sequence
Met 1-Thr190
Field of Research
Cell biology; Cardiovascular; Neuroscience; Cancer; metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 90 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
24.5 kDa
Observed Molecular Weight
30 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 8% Sucrose, 20% Glycerol, 0.02% Tween 80, 3mM TCEP, pH 8.5.
Species
Human
Available Sizes
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