Recombinant Human CD73/NT5E Protein (His Tag)
Product Specifications
Background
CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA) . The recombinant CD73 lacking GPI anchor is secreted as a monomer.
Abbreviation
CD73; NT5E
Synonyms
5'-NT;5'-Nucleotidase; CALJA; CD73; E5NT; Ecto-5'-Nucleotidase; NT; NT5; NT5E; NTE; eN; eNT
UniProt
Q6NZX3
Accession Number
AAH65937.1
Expression System
HEK293 Cells
Tag
C-His
Sequence
Trp27-Lys547
Applications
ELISA
Field of Research
Cardiovascular
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
50
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
58.8 kDa
Observed Molecular Weight
62 kDa
Formulation
2
Species
Human
Available Sizes
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