Recombinant Human HPD/4HPPD Protein (His Tag)
Product Specifications
Background
4-Hydroxyphenylpyruvate Dioxygenase (4HPPD) belongs to the 4HPPD family. 4HPPD is a key enzyme in the degradation of tyrosine, which catalyzes the second reaction in the catabolism of tyrosine the conversation of 4-hydroxyphenylpyruvate to homogentisate. 4HPPD exists in homodimer forms, which uses zinc as a cofactor to catalyze the third step in the conversion of L-phenylalanine to fumarate and acetoacetic acid. When the active 4HPPD enzyme concentration is low in the human body, it results in high levels of tyrosine concentration in the blood, which can cause mild mental retardation at birth, and degradation in vision as a patient grows older.
Abbreviation
HPD;4HPPD
Synonyms
4-Hydroxyphenylpyruvate Dioxygenase;4-Hydroxyphenylpyruvic Acid Oxidase;4HPPD; HPD; HPPDase; PPD
UniProt
P32754
Accession Number
P32754
Expression System
E.coli
Tag
N-His
Sequence
Met 1-Met393
Field of Research
Signal Transduction; Cell biology; metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
47.1 kDa
Observed Molecular Weight
40-50 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 50mM NaCl, 1mM DTT, 20% Glycerol, pH 8.0.
Species
Human
Available Sizes
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