Recombinant Human TGFB1/TGF-beta 1 Protein

Product Specifications

Background

Transforming Growth Factor β-1 (TGFβ-1) is a secreted protein which belongs to the TGF-β family. TGFβ-1 is abundantly expressed in bone; articular cartilage and chondrocytes and is increased in osteoarthritis (OA) . TGFβ-1 performs many cellular functions; including the control of cell growth; cell proliferation; cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide. TGFβ-1 may also form heterodimers with other TGFβ family members. It has been found that TGFβ-1 is frequently upregulated in tumor cells. Mutations in this gene results in Camurati-Engelmann disease.

Abbreviation

TGFB1; TGF-beta 1

Synonyms

CED; DPD1; LAP; Latency-Associated Peptide; TGF-Beta-1; TGF-beta 1; TGFB; TGFB1; Transforming Growth Factor Beta-1

UniProt

P01137

Accession Number

P01137

Expression System

CHO Stable Cells

Tag

None

Sequence

Ala279-Ser390

Applications

Cell culture

Field of Research

Signal Transduction; Cell biology; Cardiovascular; Cancer; metabolism; Stem cells

Endotoxin

< 0.01 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to inhibit the IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 4-40 pg/ml

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.