Recombinant Human/Cynomolgus VEGFA/VEGF165 Protein
Product Specifications
Background
Vascular endothelial growth factor (VEGF) ; also known as vascular permeability factor (VPF) and VEGF-A; is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF) /vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects; including mediating increased vascular permeability; inducing angiogenesis; vasculogenesis and endothelial cell growth; promoting cell migration; inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability; thus it was originally referred to as vascular permeability factor.
Synonyms
MVCD1; VEGF; VEGF165; VPF; Vascular Endothelial Growth Factor Isoform 165
UniProt
P15692
Accession Number
P15692-4
Expression System
HEK293 Cells
Tag
None
Sequence
Met 1-Arg191
Applications
Cell culture
Field of Research
Cardiovascular, Signal Transduction, Cancer, Developmental Biology, Kits, Lysates, Other, Metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) . The ED50 for this effect is typically 4-16 ng/mL.
Reconstitution
Please refer to the printed manual for detailed information.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
19.2 kDa
Observed Molecular Weight
20&22 kDa
Species
Human
Available Sizes
Documents
More Discoveries
Explore Other Products
Browse additional items from our catalog