Recombinant Human IL1R2/CD121b Protein (His Tag)

Interleukin 1 receptor; type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A) ; interleukin beta (IL1B) ; and interleukin 1 receptor; type I (IL1R1/IL1RA) ; and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate development and maintenance of the inflammatory responses; and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified; designated as IL1RI (IL1RA) and IL1RII (IL1RB) . IL1R1 contributes to IL-1 signaling; whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand binding portion comprised of three Ig-like domains; a single transmembrane region; and a short cytoplasmic domain; is expressed in a variety of cell types including B lymphocytes; neutrophils; monocytes; large granular leukocytes and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.

IL1R; Interleukin-1 receptor type; IL-1R; IL1R2; CD121b; CDw121b; IL-1R-2; IL-1RT-2; IL-1RT2; IL1R2c; IL1RB; CD121 antigen-like family member B; IL-1 type II receptor; IL-1R-beta; Interleukin-1 receptor beta; Interleukin-1 receptor type 2; Interleukin-1 receptor type I; IL-1R type II; IL-1RII

1. Measured by its ability to inhibit IL1β-dependent proliferation in D10.G4. 1 mouse helper T cells. The ED50 for this effect is typically 5. 5 μg/mL in the presence of 40 pg/mL of rhIL1β. 2. Measured by its ability to inhibit Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 500pg/mL IL1b. The EC50 for this effect is typically 20-80ng/mL.

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.