Recombinant Human NBL1/DAND1 Protein (His Tag)

The Dan (Differential screening-selected gene aberrative in neuroblastoma; also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in vertebrates. It is a founding member of the DAN family of secreted proteins; acts as an inhibitor of cell cycle progression and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN; Gremlin/DRM; Cer1 (Cerberus-related) ; Dante and PRDC (protein related to DAN and cereberus) ; and share the C-terminal cystine-knot motif. As a secreted glycoprotein; DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes; and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in regulation of the entry of cells into the S phase. In addition; DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumour suppressor gene for neuroblastoma.

Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 5-20 μg/ml in the presence of 50 ng/mL of recombinant human BMP4.

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.