Recombinant Human CD32b/FCGR2B Protein (HEK293 Cells, His Tag)

Product Specifications

Background

Processing of the N-terminal initiator methionine or formylated methionine is an essential cellular process conserved from prokaryotes to eukaryotes. The proteolytic removal of N-terminal methionine from nascent peptides is catalyzed by a family of enzymes known as methionine aminopeptidases (MetAPs) and is essential for cell growth. METAP1 and METAP2 have different substrate specificity due to the differences in both size and shape of the active sites. As a member of the M24 family of metalloproteases, METAP1 plays an important role in G (2) /M phase regulation of the cell cycle and may serve as a promising target for the discovery and development of new anticancer agents.

Synonyms

CD32; CDw32; FCG2; FCGR2B; Fc-Gamma RII-b; Fc-Gamma-RIIb; FcRII-b; IGFR2; IgG Fc Receptor II-b; Low Affinity Immunoglobulin Gamma Fc Region Receptor II-b

UniProt

P31994

Accession Number

NP_001002274.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala 46-Pro 217

Applications

ELISA

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized recombinant human CD32b at 10 μg/ml (100 μl/well) can bind human IgG2 with a linear range of 0.16-6. 4 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.