Recombinant Human IL4RA/CD124 Protein (His Tag)

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD124, also known as interleukin 4 receptor (IL4R), is a typeI transmembrane protein that can regulate IgE antibody production in B cells through binding to interleukin 4 and interleukin 13 and promote differentiation of Th2 cells through binding to interleukin 4. The membrane-bound form of CD124 can be hydrolyzed to soluble form which can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.

582J; IL4R; CD124; IL-4RA; IL4RA; IL-4 receptor subunit alpha; IL-4-binding protein; IL4-BP; IL-4R subunit alpha; IL-4R-alpha; Interleukin-4 receptor subunit alpha;582J2.1; IL-4Rα IL 4R alpha; IL4R nirs variant 1; IL4RA; Interleukin 4 receptor; Interleukin 4 receptor alpha chain; sIL4Ralpha/prot; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; Soluble interleukin-4 receptor subunit alpha

1. Immobilized human IL4R-His at 10 μg/mL (100 μl/well) can bind biotinylated human IL4. The EC50 of biotinylated human IL4 is 20.8-48.5 ng/mL. 2. Measured by its ability to inhibit IL-4 dependent proliferation of TF-1 human erthroleukemic cells. The ED50 for this effect is 4-20 ng/ml in the presence of 2 ng/ml IL-4.

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.