Recombinant Human Tie2/CD202b Protein (His Tag)
Product Specifications
Background
TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
Synonyms
CD202B; TIE-2; TIE2; VMCM; VMCM1
Accession Number
NP_000450.2
Expression System
HEK293 Cells
Tag
C-His
Sequence
Met 1-Lys 745
Applications
ELISA
Field of Research
Signal Transduction; Cardiovascular; Cancer; Developmental Biology; Stem cells
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Immobilized recombinant human Tie2 at 2 μg/ml (100 μl/well) can bind human Angiopoietin-2 at a linear range of 1. 28-160 ng/ml.
Reconstitution
Please refer to the printed manual for detailed information.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
82.0 kDa
Observed Molecular Weight
95-105 kDa
Species
Human
Available Sizes
Documents
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