Recombinant Human PSA/KLK3 Protein (His Tag)
Product Specifications
Background
KLK3 (Kallikrein 3) ; also known as Prostate Specific Antigen (PSA) ; kallikrein-related peptidase 3; Gamma-seminoprotein; is a secreted protein of the glandular kallikrein subfamily of serine proteases.KLK3 contains one peptidase S1 domain.KLK3 is a glycoprotein produced almost exclusively by the prostate gland. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
Synonyms
APS; Gamma-Seminoprotein; Hk3; KLK2A1; Kallikrein-3; P-30 Antigen; PSA; Prostate-Specific Antigen; Semenogelase; Seminin
UniProt
P07288
Accession Number
P07288
Expression System
HEK293 Cells
Tag
C-His
Sequence
Met 1-Pro 261
Applications
Enzyme
Field of Research
Signal Transduction; Tags & Cell Markers; Neuroscience; Cancer; immunology
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA) . The specific activity is > 100 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
Reconstitution
Please refer to the printed manual for detailed information.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
28.3 kDa
Observed Molecular Weight
35 kDa
Species
Human
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