Recombinant Human XPNPEP2 Protein (His Tag)

Aminopeptidase P (APP) is a hydrolase specific for N-terminal imido bonds; which are common to several collagen degradation products; neuropeptides; vasoactive peptides; and cytokines. A membrane-bound and soluble form of this enzyme (XPNPEP2) have been identified as products of two separate genes. XPNPEP2; the X-linked gene that encodes membranous aminopeptidase P (APP) ; has been reported to associate with APP activity. The membrane aminopeptidase P (XPNPEP2) is largely limited in distribution to endothelia and brush border epithelia. APP and XPNPEP2 contain homologous blocks of sequence common to members of the "pita bread-fold" protein family; of which Escherichia coli methionine aminopeptidase is the prototype. The C-2399A variant in XPNPEP2 is associated with reduced APP activity and a higher incidence of AE-ACEi. XPNPEP2 mRNA was detected in fibroblasts that carry the translocation; suggesting that this gene at least partially escapes X inactivation. XPNPEP2 is a candidate gene for premature ovarian failure (POF) .

Aminoacylproline Aminopeptidase; Membrane-Bound APP; Membrane-Bound AmP; Membrane-Bound Aminopeptidase P; X-Pro Aminopeptidase 2; XPNPEP2; Xaa-Pro Aminopeptidase 2; mAmP

Measured by its ability to cleave the fluorogenic peptide substrate, H-Lys (2-Aminobenzoyl) Pro-Pro-pNitroanilide (K (Abz) PP-pNA) . The specific activity is > 300 pmoles/min/μg.

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.