Recombinant Human STAT1 Protein (His &GST Tag)

STAT1 is a member of the STAT protein family. In response to cytokines and growth factors; STAT family members are phosphorylated by the receptor associated kinases; and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 can be activated by various ligands; including interferon-alpha; interferon-gamma; EGF; PDGF and IL6. It is a signal transducer and transcription activator that mediates cellular responses to interferons (IFNs) ; cytokine KITLG/SCF and other cytokines and growth factors. The phosphorylated STATs dimerize; associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor; that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes; which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma) ; STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF) ; migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes; inducing a cellular antiviral state. STAT1 becomes activated in response to KITLG/SCF and KIT signaling and may mediate cellular responses to activated FGFR1; FGFR2; FGFR3 and FGFR4. Defects in STAT1 can cause STAT1 deficiency complete and familial candidiasis type 7.

CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT1; STAT91; Signal Transducer and Activator of Transcription 1-Alpha/Beta; Transcription Factor ISGF-3 Components p91/p84

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.