Recombinant Human Alkaline Phosphatase/ALPI Protein (His Tag)
Product Specifications
Background
ALPI encodes for intestinal phosphatase alkaline; a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.
Synonyms
IAP
UniProt
P09923
Accession Number
P09923
Expression System
HEK293 Cells
Tag
C-His
Sequence
Met 1-Asp 503
Applications
Enzyme
Field of Research
Signal Transduction; Tags & Cell Markers; Cell biology; Cancer; immunology; metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP) . The specific activity is > 1000 pmoles/min/μg.
Reconstitution
Please refer to the printed manual for detailed information.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Product Datasheet
https://789.bio/ea/8og4oo
Product MSDS
https://789.bio/eb/XfzfbH
Calculated Molecular Weight
54.0 kDa
Observed Molecular Weight
66 kDa
Species
Human
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