Recombinant Human PDGF-BB Protein (His Tag)

Product Specifications

Background

Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.

Synonyms

Becaplermin; PDGF Subunit B; PDGF-2; PDGF2; PDGFB; Platelet-Derived Growth Factor B Chain; Platelet-Derived Growth Factor Beta Polypeptide; Platelet-Derived Growth Factor Subunit B; Proto-Oncogene c-Sis; SIS

UniProt

P01127

Accession Number

NP_148937.1

Expression System

Yeast

Tag

N-His

Sequence

Ser82-Thr190

Applications

ELISA

Field of Research

Signal Transduction; Cardiovascular; Cancer; Developmental Biology; metabolism

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human PDGFB at 10μg/mL (100μL/well) can bind human PDGFRβ-Fch, the EC50 of human PDGFRβ-Fch is 0.05-0.2 μg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.