Recombinant Human EGFR/ErbB1 Protein (aa 668-1210, His&GST Tag)
Product Specifications
Background
As a member of the epidermal growth factor receptor (EGFR) family; EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF; amphiregulin; TGF-α ; betacellulin; etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation; survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization; the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK; PI3K/PKB and STAT) . In addition; EGFR signaling also has been shown to exert action on carcinogenesis and disease progression; and thus EGFR protein is proposed as a target for cancer therapy currently.
Synonyms
ERBB; ERBB1; HER1; NISBD2; PIG61; mENA
UniProt
P00533
Accession Number
NP_005219
Expression System
Baculovirus-Insect Cells
Tag
N-His-GST
Sequence
Met 668-Ala 1210
Applications
Enzyme
Field of Research
Signal Transduction; Cancer
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 85 % as determined by reducing SDS-PAGE.
Bioactivity
The specific activity was determined to be 105 nmol/min/mg using Poly (Glu:Tyr) 4:1 as substrate.
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
89.1 kDa
Formulation
Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4
Species
Human
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