Recombinant Human Bruton Tyrosine Kinase/BTK Kinase Protein (His Tag)
Product Specifications
Background
Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol (3,4,5) -trisphosphate (PIP3) . After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia (Bruton's agammaglobulinemia) . BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.
Synonyms
AGMX1; AT; ATK; BPK; IMD1; PSCTK1; XLA
UniProt
Q06187
Accession Number
NP_000052.1
Expression System
Baculovirus-Insect Cells
Tag
C-His
Sequence
Met 1-Ser 659
Applications
Enzyme
Field of Research
Signal Transduction; immunology
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 85 % as determined by reducing SDS-PAGE.
Bioactivity
The specific activity was determined to be 115 nmol/min/mg using Poly (Glu, Tyr) 4:1 peptide as substrate.
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
77.8 kDa
Formulation
Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.0
Species
Human
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