Recombinant Human ERN1/IRE1 Protein (aa 465-977, His & GST Tag)
Product Specifications
Background
The encoded protein contains two functional catalytic domains; a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR) . The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins; regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.
Synonyms
IRE1; IRE1P; IRE1a; hIRE1p
UniProt
O75460
Accession Number
O75460-1
Expression System
Baculovirus-Insect Cells
Tag
N-His-GST
Sequence
Pro 465-Leu 977
Applications
Enzyme
Field of Research
Signal Transduction
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 80 % as determined by reducing SDS-PAGE.
Bioactivity
The specific activity was determined to be 5 nmol/min/mg using MBP as substrate
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
86.0 kDa
Observed Molecular Weight
90 kDa
Formulation
Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
Species
Human
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