Recombinant Human MAPKAPK5 Protein (His & GST Tag)
Product Specifications
Background
MAPKAPK5 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family. MAPKAPK5 has significant sequence homology to mitogen-activated protein kinase (MAPK) -activated protein kinase (MAPKAPK) . It is widely distributed. MAPKAPK5 can be phosphorylated by extracellular-regulated kinase (ERK), and p38 kinase but not by c-jun N-terminal kinase (JNK) in vitro.Recombinant GST-MAPKAPK5 protein can phosphorylate a peptide derived from the regulatory light chain of myosin II. Phosphorylation of MAPKAPK5 by ERK and p38 kinase increased its activity by 9 and 15 fold respectively. Taken together, these data suggest that MAPKAPK5 is a novelin vitrosubstrate for ERK and p38 kinase. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. MAPKAPK5 also mediates stress-induced small heat shock protein 27 phosphorylation.
Synonyms
MAPKAP-K5; MK-5; MK5; PRAK
UniProt
Q8IW41
Accession Number
NP_003659.2
Expression System
Baculovirus-Insect Cells
Tag
N-His-GST
Sequence
Met 1-Gln 471
Field of Research
Cancer
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 76 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
82.0 kDa
Observed Molecular Weight
75 kDa
Formulation
Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol
Species
Human
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