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Recombinant Human Flavokinase/RFK Protein (His Tag)

Product Specifications

Background

Flavokinase is a member of the transferases family, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. Flavokinase is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. It has been proposed that TNF, through the activation of the flavokinase gene, enhances the incorporation of FAD in NADPH oxidase enzymes, which is a critical step for the assembly and activation of NADPH oxidase.

Synonyms

RIFK

UniProt

Q969G6

Accession Number

Q969G6

Expression System

E.coli

Tag

N-His

Sequence

Met 1-His 155

Field of Research

Signal Transduction; metabolism

Endotoxin

Please contact us for more information.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

19.5 kDa

Observed Molecular Weight

20 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 10% glycerol, pH 8.0

Species

Human

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