Recombinant Human PFK1/PFKM Protein (His & GST Tag)
Product Specifications
Background
PFK1, also known as PFKM, is a regulatory glycolytic enzyme. PFK1 converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. It is a muscle-type isozyme. There are three phosphofructokinase isozymes in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Mutations in PFK1 gene have been related with glycogen storage disease type VII, also identified as Tarui disease.
Synonyms
6-phosphofructokinase; ATP-PFK; GSD7; PFK-1; PFK1; PFKA; PFKM; PFKX; PPP1R122; Phosphofructo-1-kinase isozyme A; Phosphofructokinase 1; Phosphohexokinase; muscle type
UniProt
P08237
Accession Number
P08237-1
Expression System
Baculovirus-Insect Cells
Tag
N-His-GST
Sequence
Thr 2-Val 780
Field of Research
Signal Transduction, Cancer, Metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 90 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
112.9 kDa
Observed Molecular Weight
113 kDa
Formulation
Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol
Species
Human
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