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Recombinant Human SPHK1/Sphingosine Kinase 1 Protein (His&GST Tag)

Selected Variant

PKSH030316

Size: 50 µg

Product Specifications

Background

SPHK1; also known as sphingosine Kinase 1; catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P) . S1P exhibits a broad spectrum of biological activities including cell proliferation; survival; migration; cytoskeletal organization; and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory; antiapoptotic; and immune processes.

Synonyms

SPHK; SPHK1; SPK

UniProt

Q9NYA1

Accession Number

Q9NYA1-1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 1-Leu 384

Field of Research

Signal Transduction, Cancer, Metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

70.3 kDa

Observed Molecular Weight

64 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 8.0

Species

Human

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