Recombinant Human SPHK1/Sphingosine Kinase 1 Protein
Product Specifications
Background
SPHK1; also known as sphingosine Kinase 1; catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P) . S1P exhibits a broad spectrum of biological activities including cell proliferation; survival; migration; cytoskeletal organization; and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory; antiapoptotic; and immune processes.
Synonyms
SPHK; SPHK1; SPK
UniProt
Q9NYA1
Accession Number
Q9NYA1-1
Expression System
Baculovirus-Insect Cells
Tag
None
Sequence
Met 1-Leu 384
Applications
Enzyme
Field of Research
Signal Transduction, Cancer, Metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 85 % as determined by reducing SDS-PAGE.
Bioactivity
The specific activity was determined to be 1028 nmol/min/mg using Sphingosine Kinase Substrate as substrate.
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
42.7 kDa
Observed Molecular Weight
46 kDa
Formulation
Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0
Species
Human
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