Recombinant Human AMPK (G1/B1/A1) Heterotrimer Protein
Product Specifications
Synonyms
AMPK; AMPKa1
UniProt
P54619-1; Q9Y478-1; Q13131-1
Accession Number
P54619-1&Q9Y478-1&Q13131-1
Expression System
Baculovirus-Insect Cells
Tag
N-His-GST
Sequence
Met 1-Pro 331&Met 1-Ile 270&Met 1-Gln 559
Applications
Enzyme
Field of Research
Signal Transduction; Cardiovascular; Cancer; metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 94 % as determined by reducing SDS-PAGE.
Bioactivity
The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
38&30&92 kDa
Observed Molecular Weight
38&40&95 kDa
Formulation
Supplied as sterile solution of 50mM Tris, 200mM NaCl, 2mM GSH, 1mM EDTA, 1mM DTT, 10% glycerol, pH 7.4
Citation 01
https://febs.onlinelibrary.wiley.com/doi/abs/10.1002/1878-0261.13211
Species
Human
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