Recombinant Human WISP1 protein (His tag)
Product Specifications
Background
Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X (L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. Expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Isoform 2 is expressed predominantly in scirrhous gastric carcinoma and, weakly in placenta. Overexpression is associated with several cancers including breast cancer and colon tumors. Isoform 2 is overexpressed in scirrhous gastric carcinoma.
Abbreviation
WISP1
Synonyms
CCN family member 4; CCN4; WISP-1; WISP1; WNT1-inducible-signaling pathway protein 1; Wnt-1-induced secreted protein
UniProt
O95388
Accession Number
O95388
Expression System
HEK293 Cells
Tag
C-His
Sequence
Met1-Asn367
Field of Research
Epigenetics and Nuclear Signaling
Endotoxin
< 1.0 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Product Datasheet
https://789.bio/ea/lwhxdr
Product MSDS
https://789.bio/eb/b1GCK8
Calculated Molecular Weight
40.3 kDa
Observed Molecular Weight
60 kDa
Species
Human
Available Sizes
Curated Selection
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