Recombinant Human Apolipoprotein (a) /Lp (a) protein (His tag)

Product Specifications

Background

Apo (a) is the main constituent of lipoprotein (a) (Lp (a) ) . It has serine proteinase activity and is able of autoproteolysis. Inhibits tissue-type plasminogen activator 1. Lp (a) may be a ligand for megalin/Gp 330.Apo (a) is known to be proteolytically cleaved, leading to the formation of the so-called mini-Lp (a) . Apo (a) fragments accumulate in atherosclerotic lesions, where they may promote thrombogenesis. O-glycosylation may limit the extent of proteolytic fragmentation. Homology with plasminogen kringles IV and V is thought to underlie the atherogenicity of the protein, because the fragments are competing with plasminogen for fibrin (ogen) binding.

Abbreviation

Apolipoprotein; Lp

Synonyms

APOA; Apo (a) ; Apolipoprotein (a) ; LPA; Lp (a)

UniProt

P08519

Accession Number

P08519

Expression System

HEK293 Cells

Tag

C-His

Sequence

Asp1719-Arg2038

Field of Research

Cardiovascular, Neuroscience, Signal Transduction, Metabolism

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.