PH20 Hyaluronidase Recombinant Protein, Tag Free
PH20 Hyaluronidase Recombinant Protein, Tag Free
Product Specifications
Product Name Alternative
SPAM1, PH-20, HYAL3, HYA1, HYAL1, HYAL5, SPAG15
UniProt
P38567
Tag
No Tag
Source
HEK293 cells
Purity
>95% as determined by SDS-PAGE and >95% as determined by SEC-MALS.
Bioactivity
The activity of PH20 is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay) . The specific activity is >55000 U/mg. (Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH5.35 at 37 ̊C in a 2.0 mL reaction mixture) (QC tested) .
Form
Lyophilized
Molecular Weight
52.6 kDa
Storage Conditions
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This recombinant protein can be used for WB. For research use only.
Tested Applications
WB
NCBI Accession Number
AAH26163
Preservative
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.5 with trehalose as protectant.
AA Sequence
Leu 36 - Ser 490
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