Recombinant HIF1 beta Monoclonal Antibody

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1) . The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2) . HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4) . Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7) .HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8) . Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8) .

ArnT; ARNT protein; ARNT; Aryl hydrocarbon receptor nuclear translocator; bHLHe2; Class E basic helix-loop-helix protein 2; Dioxin receptor; Dioxin receptor nuclear translocator; Drnt; HIF 1 beta; HIF 1beta; HIF-1-beta; HIF1-beta; HIF1B; HIF1beta; Hypoxia Inducible Factor 1; Hypoxia inducible factor 1 beta; Hypoxia-inducible factor 1-beta; nuclear translocator; Tango