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Recombinant Human Leptin protein (LEP) (Active)

Recombinant Human Leptin protein (LEP) (Active)

Product Specifications

Product Name Alternative

Obese protein

UniProt

P41159

Tag

NO-tagged

Field of Research

Metabolism Research

Endotoxin

Less than 1 EU/g of rHuLeptin as determined by LAL method.

Purity

>97% as determined by SDS-PAGE and HPLC.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 105 IU/mg.

Form

Lyophilized from a 0.2 m filtered concentrated solution in 50 mm PB, pH 3.5, with 0.02 % Tween-20.

Molecular Weight

16.0 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

For research use only.

AA Sequence

VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC

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