Recombinant TriMethyl-Histone H3 Monoclonal Antibody
Product Specifications
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Abbreviation
TriMethyl-Histone H3
UniProt
P68431
Host
Rabbit
Reactivity
Human; Rat
Immunogen
A synthetic methyl-peptide corresponding to residues surrounding Lys27 of human Histone H3
Target
H3 histone family; member A; H3/A; H31; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1; H3a; Histone cluster 1; H3a; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
Clonality
Monoclonal
Clone
R01-8I4
Conjugation
Unconjugated
Applications
WB; IHC-P; IF
Field of Research
Cancer; Epigenetics and Nuclear Signaling
Purification
Affinity Purified
Concentration
300 μg/mL
Dilution
WB 1:500-1:1000; IHC 1:50-1:100; IF 1:50-1:100
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
15kDa
Observed Molecular Weight
15kDa
Isotype
IgG
Available Sizes
Documents
Curated Selection
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