Recombinant Cytochrome C Monoclonal Antibody
Product Specifications
Background
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.
Abbreviation
Cytochrome C
UniProt
P99999
Host
Rabbit
Reactivity
Human; Mouse; Rat
Immunogen
A synthetic peptide of human Cytochrome C
Target
Cytochrome C; Somatic; CYC; Cytochrome C; THC4; HCS
Clonality
Monoclonal
Clone
R09-6H3
Conjugation
Unconjugated
Applications
WB; IHC-P
Field of Research
Cancer; Cell Biology; Cardiovascular; Metabolism; Signal Transduction
Purification
Affinity Purified
Concentration
300 μg/mL
Dilution
WB 1:1000-1:2000; IHC 1:50-1:100
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
12kDa
Observed Molecular Weight
14kDa
Citation 01
https://onlinelibrary.wiley.com/doi/abs/10.1111/and.14185
Isotype
IgG
Available Sizes
Documents
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