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Recombinant Vimentin Monoclonal Antibody

Product Specifications

Background

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein,55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

Abbreviation

Vimentin

UniProt

P08670

Host

Rabbit

Reactivity

Human; Mouse; Rat; Hamster

Immunogen

A synthetic peptide of human Vimentin

Target

CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME; Vimentin

Clonality

Monoclonal

Clone

R02-8C4

Conjugation

Unconjugated

Applications

WB; IHC-P; IF

Field of Research

Cancer; Developmental Biology; Neuroscience; Signal Transduction; Stem Cells

Purification

Affinity Purified

Concentration

300 μg/mL

Dilution

WB 1:500-1:1000; IHC 1:50-1:100; IF 1:50-1:100

Buffer

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

54kDa

Observed Molecular Weight

54kDa

Isotype

IgG

Available Sizes

Frequently Asked Questions

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