BORA Polyclonal Antibody
Product Specifications
Background
Aurora related kinase-1 (ARK-1) is a centrosome-associated serine/threonine kinase that regulates centrosome separation, bipolar spindle assembly and chromosome segregation during mitosis. Bora (protein aurora borealis) is a 559 amino acid protein that activates ARK-1. Bora is localized to the nucleus until mitosis is initiated, when it then translocates to the cytoplasm. This translocation is dependent on activated Cdc2, which releases Bora to bind and activate ARK-1 in the cytoplasm. Plk (polo-like kinase) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation on its N-terminus by ARK-1. It is through this mechanism that Bora and ARK-1 control cellular mitotic entry. Downregulation of the gene encoding Bora results in multipolar spindles in mitosis, a phenomenon that is also observed when ARK-1 function is blocked.
Abbreviation
BORA
UniProt
Q6PGQ7
Host
Rabbit
Reactivity
Human
Immunogen
Synthetic peptide of human BORA
Target
Aurora borealis; bora; bora; aurora kinase A activator; BORA; C13orf34; Chromosome 13 open reading frame 34 ; FLJ22624; HsBora; Protein aurora borealis; RP11-342J4.2
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
WB; IHC
Field of Research
Cancer; Cell Biology; Signal transduction
Purification
Antigen affinity purification
Concentration
1.8 mg/mL
Dilution
WB 1:500-1:2000; IHC 1:50-1:300
Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
61 kDa
Observed Molecular Weight
Refer to figures
Isotype
IgG
Available Sizes
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