NGEF Polyclonal Antibody
Product Specifications
Background
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity) .
Abbreviation
NGEF
UniProt
Q8N5V2
Host
Rabbit
Reactivity
Human; Mouse; Rat
Immunogen
Fusion protein of human NGEF
Target
ARHGEF27; Eph receptor-interacting exchange protein; Eph-interacting exchange protein; EPHEXIN; Ephexin-1; Guanine nucleotide exchange factor; neuronal; Neuronal guanine nucleotide exchange factor; Ngef; NGEF
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
WB; IHC
Field of Research
Neuroscience; Signal transduction
Purification
Antigen affinity purification
Concentration
0.6 mg/mL
Dilution
WB 1:500-1:2000; IHC 1:30-1:150
Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
82 kDa
Observed Molecular Weight
Refer to figures
Isotype
IgG
Available Sizes
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