Phospho-AKT1 (Ser473) Monoclonal Antibody
Product Specifications
Background
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Abbreviation
Phospho-AKT1 (Ser473)
UniProt
P31749
Host
Mouse
Reactivity
Human
Immunogen
Synthetic Peptide of Phospho-Akt (S473)
Target
AKT 1; AKT; AKT1; AKT1; MGC99656; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha
Clonality
Monoclonal
Clone
Clone:7F9
Conjugation
Unconjugated
Applications
WB; IHC-p
Field of Research
Cancer; Epigenetics and Nuclear Signaling; Metabolism; Signal Transduction
Purification
Protein A purification
Concentration
1 mg/mL
Dilution
WB 1:1000-2000; IHC 1:100-200
Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Observed Molecular Weight
60kDa
Citation 01
https://www.liebertpub.com/doi/abs/10.1089/ars.2019.7923
Isotype
IgG
Available Sizes
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