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Flag-Tag Monoclonal Antibody

Product Specifications

Background

FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This antibody detects FLAG tag in most applications.

Abbreviation

Flag-Tag

Host

Mouse

Reactivity

All

Immunogen

Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin.

Target

DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag

Clonality

Monoclonal

Clone

3H9

Conjugation

Unconjugated

Applications

WB; IF; IP; FCM

Purification

Antigen Affinity Purification

Concentration

1 mg/mL

Dilution

WB 1:5000-10000; IF 1:500-2000; IP 3ug/sample; FCM 0.2ug

Buffer

PBS with 0.02% sodium azide,1% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

45kDa

Observed Molecular Weight

45kDa

Citation 01

https://pubmed.ncbi.nlm.nih.gov/36871040/

Citation 02

https://pubmed.ncbi.nlm.nih.gov/35194032/

Citation 03

https://pubmed.ncbi.nlm.nih.gov/35603194/

Citation 04

https://pubmed.ncbi.nlm.nih.gov/37010434/

Citation 05

https://pubmed.ncbi.nlm.nih.gov/32595669/

Citation 07

https://pubmed.ncbi.nlm.nih.gov/36122636/

Citation 08

https://pubmed.ncbi.nlm.nih.gov/32188757/

Citation 09

https://pubmed.ncbi.nlm.nih.gov/32470978/

Citation 10

https://pubmed.ncbi.nlm.nih.gov/35746667/

Citation 11

https://pubmed.ncbi.nlm.nih.gov/33310083/

Citation 12

https://pubmed.ncbi.nlm.nih.gov/32745481/

Isotype

IgG2a

Available Sizes

Curated Selection

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