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Beta actin Monoclonal Antibody

Product Specifications

Background

Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells, and commonly used as a loading control for western blot and as a control for qPCR.

Abbreviation

Beta actin

UniProt

Q7ZVF9

Host

Mouse

Reactivity

Human; Mouse; Rat; Zebrafish

Immunogen

Recombinant Human Beta actin protein

Target

ACTB; Actin beta; Actx; Actin cytoplasmic 1; b actin; A26C1A

Clonality

Monoclonal

Clone

2B2

Conjugation

Unconjugated

Applications

WB; IF

Purification

Antigen Affinity Purification

Concentration

1 mg/mL

Dilution

WB 1:1000-2000; IF 1:100-200

Buffer

PBS with 0.02% sodium azide,1% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Product Datasheet

https://789.bio/ea/4l9gvh

Product MSDS

https://789.bio/eb/vbbL00

Calculated Molecular Weight

43kDa

Observed Molecular Weight

43kDa

Citation 01

https://link.springer.com/article/10.1007/s00018-021-04054-8

Citation 02

https://bpspubs.onlinelibrary.wiley.com/doi/abs/10.1111/bph.15413

Citation 03

https://www.sciencedirect.com/science/article/pii/S0753332222012732

Citation 04

https://www.mdpi.com/2072-6643/14/19/4120

Citation 05

https://www.sciencedirect.com/science/article/pii/S0147651319300594

Citation 06

https://www.mdpi.com/2227-9059/10/1/181

Citation 07

https://www.mdpi.com/1806946

Citation 08

https://pubmed.ncbi.nlm.nih.gov/37010434/

Citation 09

https://www.mdpi.com/1422-0067/22/15/8094

Citation 10

https://www.mdpi.com/1422-0067/23/9/5128

Citation 11

https://www.mdpi.com/1019868

Citation 12

https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.202101816RR

Isotype

IgG2a

Available Sizes

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