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GAPDH Monoclonal Antibody

Product Specifications

Background

GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR.

Abbreviation

GAPDH

UniProt

Q5XJ10

Host

Mouse

Reactivity

Human; Mouse; Rat ; Bovine; Caprine; Guinea pig; Rabbit; Porcine

Immunogen

Recombinant Human GAPDH protein expressed by E.coli

Target

Aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component

Clonality

Monoclonal

Clone

3B3

Conjugation

Unconjugated

Applications

WB; IF

Field of Research

Cancer; Metabolism; Signal Transduction

Purification

Antigen Affinity Purification

Concentration

1 mg/mL

Dilution

WB 1:5000-10000

Buffer

PBS with 0.02% sodium azide,1% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

36 kDa

Observed Molecular Weight

36 kDa

Citation 01

https://pubmed.ncbi.nlm.nih.gov/35194032/

Citation 02

https://www.sciencedirect.com/science/article/pii/S2452199X21004874

Citation 03

https://pubmed.ncbi.nlm.nih.gov/36512628/

Citation 04

https://www.sciencedirect.com/science/article/pii/S2590006422001405

Citation 05

https://www.sciencedirect.com/science/article/pii/S0142961219303473

Citation 06

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295061/

Citation 07

https://www.sciencedirect.com/science/article/pii/S0753332223000823

Citation 08

https://www.sciencedirect.com/science/article/pii/S0753332222012331

Citation 09

https://www.nature.com/articles/s41401-022-01045-8

Citation 10

https://www.mdpi.com/2161010

Citation 11

https://www.frontiersin.org/articles/10.3389/fonc.2021.727605/full

Citation 12

https://www.sciencedirect.com/science/article/pii/S0891584920313022

Isotype

IgG1

Available Sizes

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