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PCNA Monoclonal Antibody

Product Specifications

Background

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics. PCNA gene is often stably and constitutively expressed in most tissues and cells, it is commonly used as a loading control for western blot and other test.

Abbreviation

PCNA

UniProt

P12004

Host

Mouse

Reactivity

Zebrafish

Immunogen

Recombinant Human PCNA protein expressed by E.coli

Target

ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; OTTHUMP00000030189; OTTHUMP00000030190; Pcna/cyclin; PCNAR; Polymerase delta accessory protein; Proliferating cell nuclear antigen; wu:fa28e03; wu:fb36g03

Clonality

Monoclonal

Clone

8B12

Conjugation

Unconjugated

Applications

WB; IF; IHC

Field of Research

Cancer; Cell Biology; Epigenetics and Nuclear Signaling; Tags and Cell Markers

Purification

Antigen Affinity Purification

Concentration

1 mg/mL

Dilution

WB 1:1000-2000

Buffer

PBS with 0.02% sodium azide,1% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

30kDa

Observed Molecular Weight

36kDa

Isotype

IgG1

Available Sizes

Frequently Asked Questions

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