GRP78 Polyclonal Antibody
Product Specifications
Background
GRP78 (Glucose-regulated protein 78 kDa; also BiP and HSPA5) is a 72 kDa member of the heat shock protein 70 family of proteins. Intracellularly, GRP78 is an endoplasmic reticulum chaperone that participates in protein folding; extracellularly, it induces IL-10 production from T cells and interacts with Cripto to block TGF-beta signaling. Human GRP78 precursor is 654 amino acids (aa) in length. It contains an 18 aa signal sequence and a 636 aa mature region that shows a hydantoinase A region (aa 145245) and a C-terminal KDEL motif that is present on intracellular GRP78, but absent on secreted GRP78. There is alternative splicing in the signal sequence (aa 110), and multiple single aa substitituion. Over aa 1654, human GRP78 is more than 97% aa identical to mouse and rat GRP78.
Abbreviation
GRP78
UniProt
P11021
Host
Rabbit
Reactivity
Human; Mouse; Rat
Immunogen
Recombinant Human GRP78 protein expressed by E.coli.
Target
BiP; GRP-78; HSP70 family protein 5; Heat shock protein family A member 5; Immunoglobulin heavy chain-binding protein; HSPA5
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
WB
Field of Research
Cancer; Tags and Cell Markers
Purification
Antigen Affinity Purification
Concentration
1 mg/mL
Dilution
WB 1:500-1000
Buffer
PBS with 0.02% sodium azide,1% protective protein and 50% glycerol, pH7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
72kDa
Observed Molecular Weight
78kDa
Citation 01
https://www.spandidos-publications.com/10.3892/ijmm.2019.4432
Isotype
IgG
Available Sizes
Documents
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