Beta actin Polyclonal Antibody

Product Specifications

Background

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle) . Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1 (found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Abbreviation

ACTBB

UniProt

Q7ZVF9

Host

Rabbit

Reactivity

Human; Mouse; Rat

Immunogen

Recombinant Zebrafish Actin, cytoplasmic 2 protein

Target

ACTB; Actin beta; Actx; Actin cytoplasmic 1; b actin; A26C1A

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Isotype; Tags and Cell markers; Signal Transduction

Purification

Antigen Affinity Purification

Concentration

0.85mg/mL

Dilution

WB 1:3000-1:30000; IHC 1:100-1:300

Buffer

PBS with 0.02% sodium azide,1% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

41 kDa

Observed Molecular Weight

41 kDa

Citation 01

https://www.sciencedirect.com/science/article/pii/S2162253120301724

Citation 02

https://pubs.rsc.org/en/content/articlelanding/2022/tb/d2tb00114d

Citation 03

https://www.mdpi.com/2073-4409/10/10/2647

Citation 04

https://www.mdpi.com/2072-6694/13/9/2066

Citation 05

https://www.mdpi.com/2073-4409/10/5/1013

Citation 06

https://www.mdpi.com/1905846

Citation 07

https://www.mdpi.com/1905046

Citation 08

https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.7293

Citation 09

https://www.sciencedirect.com/science/article/pii/S1567576922007597

Citation 10

https://cancerci.biomedcentral.com/articles/10.1186/s12935-020-1132-3

Citation 11

https://www.sciencedirect.com/science/article/pii/S0944711321003883

Citation 12

https://academic.oup.com/jpp/advance-article-abstract/doi/10.1093/jpp/rgac065/6726478

Isotype

IgG

Available Sizes

Curated Selection

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